New technology developed for single-cell analysis

New technology developed for single-cell analysis

New technology developed for single-cell analysis

New technology developed for single-cell analysis

The single-cell electrorotation microfluidic device uses an electric field to investigate the properties of the cell.

Emily Oswald / Texas A&M Engineering

The ability to analyze the properties of individual cells is important for a wide range of life science applications, from diagnosing diseases and developing better therapeutics to pathogenic bacteria and cells for bioproduction applications.

However, accurate analysis of individual cells is a challenge, especially when it comes to the biophysical properties of the cell, even with large property variation between cells in the same cell population. Due to the presence of rare cell types within.

Addressing this need, Arum HanoTexas Instruments Professor II in the Department of Electrical and Computer Engineering at Texas A&M University, along with his graduate students and postdoctoral researchers, has developed a new technique that can accurately analyze cell properties through the use of a single-cell electrorotation microfluidic device. Is. Which uses an electric field to investigate the properties of a cell.

The technique works by first using an electric field to hold a single cell in a microfluidic device, then applying a rotating electric field to rotate the trapped single cell, and then measuring the speed of rotation. By knowing the input electric field parameters and analyzing the rotation speed, precise analysis of the dielectric properties of a single cell becomes possible.

“By knowing how much force is applied and how fast the cell spins, you can extract many basic biophysical properties of cells,” Han said.

There have been previous attempts to accomplish this, but this technique is the most accurate in measuring these properties due to its ability to apply a high-frequency electric field (up to 100 MHz) and its use of an eight-electrode-pair design. Is. Trap a single cell together and apply a rotational force to the trapped cell.

research team’s findings Featured on the cover of the June 2022 issue of the Journal biomedical microdevice,

This technology has been thoroughly developed and applied to many different cell analysis applications. After successfully demonstrating that the analysis can be completed accurately on one cell at a time, Yuwen Li, a graduate student in Han’s lab and lead author of the work, is now leading the effort to further develop the technology so that It can be executed at a very high speed and against multiple cells at once.

Other contributors to this research are postdoctoral researcher Cain Huang and research scientist Songyi Han of the Department of Electrical and Computer Engineering.

The project was funded through a cooperative agreement with the US Army Combat Capabilities Development Command Army Research Laboratory, which was facilitated through the Texas A&M Engineering Experiment Station.

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